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protein molecular weight markers  (TaKaRa)


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    Structured Review

    TaKaRa protein molecular weight markers
    Protein Molecular Weight Markers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+molecular+weight+markers/Protein+Molecular+Weight+Marker/pm42085683-393-2-6
    Average 94 stars, based on 512 article reviews
    protein molecular weight markers - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: Intercellular signal transduction within the mother cell compartment during Bacillus subtilis sporulation.
    Article Snippet: .. 637 a; Protein molecular weight markers (Takara Bio Inc.), including pig myosin (200 kDa), E. coli β-638 galactosidase (116 kDa), and rabbit muscle phosphorylase B (97.2 kDa), were added to sterile distilled 639 water (SDW) or B. subtilis culture supernatant at T2 (Culture) to the concentration of 0.9 μg/μl. ..

    Article Title: Thermostable Whole-Cell Biocatalysts Enable Sustainable and Practical Synthesis of N -Acetylneuraminic Acid.
    Article Snippet: N-Acetylneuraminic acid (Neu5Ac), a naturally occurring monosaccharide, has diverse applications in food, cosmetics, and pharmaceuticals.. A two-enzyme cascade involving N-acetylglucosamine-2-epimerase (AGE) and N-acetylneuraminic acid aldolase (NanA) offers a viable strategy for the synthesis of Neu5Ac from N-acetylglucosamine (GlcNAc) and pyruvate, although improving yield and substrate-to-product conversion remains a challenge.. Here, thermostable AGE and NanA with high specific activity and a high soluble expression level were identified via sequence similarity networks.

    Article Title: Enhanced extracellular production of maltotetraose amylase from Pseudomonas saccharophila in Bacillus subtilis through regulatory element optimization
    Article Snippet: The codon-optimized gene encoding Mta (GenBank accession number E02567.1) from Pseudomonas saccharophila was synthesized by Synbio Technologies (Suzhou, China). .. The PrimeSTAR HS DNA polymerase, In-Fusion HD Cloning Kit, E. coli HST08 Premium Competent Cells, DNA and protein molecular weight markers were purchased from Takara (Dalian, China). .. Maltotetraose standard (HPLC ≥ 98%) was purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China).

    Article Title: Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33.
    Article Snippet: Catalase, which catalyzes the decomposition of H2O2 to H2O and O2, is widely used to reduce H2O2 in industrial applications, such as in food processing, textile dyeing and wastewater treatment.. In this study, the catalase (KatA) from Bacillus subtilis was cloned and expressed in the yeast Pichia pastoris X-33.. The effect of the promoter in the expression plasmid on the activity level of the secreted KatA protein was also studied.

    Article Title: High-Level Expression and Biochemical Characterization of a Maltotetraose Amylase in Pichia pastoris X-33 for Maltotetraose Production.
    Article Snippet: Maltotetraose amylase, which catalyzes the hydrolysis of amylaceous polysaccharides into maltooligosaccharides with maltotetraose as the main product, is extensively used in the food industry.. However, the lack of efficient expression system for maltotetraose amylase has hampered its production and application.. In this study, high-level production of a maltotetraose amylase mutant (referred to as Pp-Mta∆CBM) from Pseudomonas saccharophila was achieved in Pichia pastoris X-33.

    Article Title: Blocking mutant IDH1 phosphorylation triggers APC/C CDH1-dependent ubiquitination in mitotic cells.
    Article Snippet: IDH1 mutation occurs early in glioma development; thus, mutant IDH1-specific inhibitors are being developed as glioma therapy.. But, recent reports suggest that mutant IDH1 inhibitors treatments result in loss of therapeutic vulnerabilities and makes cells resistant to anticancer agents.. To overcome resistance, the new paradigm in drug discovery is to develop molecules that can degrade oncogenes by harnessing cellular ubiquitination machinery.

    Article Title: Heterologous expression and activity of α-helical antimicrobial peptide SW in Bacillus subtilis
    Article Snippet: The antimicrobial peptide Cecropin P1 (CP1) was isolated from the pig-intestinal parasitic nematode Ascaris lumbricoides and had potent antimicrobial activity.. However, its cytotoxicity is high.. Herein, Using CP1 as the parent peptide, we designed a derivative peptide SW by replacing amino acids based on the structure and function relationship of antimicrobial peptides (AMPs) to reduce the toxicity of CP1.

    Sterility:

    Article Title: Intercellular signal transduction within the mother cell compartment during Bacillus subtilis sporulation.
    Article Snippet: .. 637 a; Protein molecular weight markers (Takara Bio Inc.), including pig myosin (200 kDa), E. coli β-638 galactosidase (116 kDa), and rabbit muscle phosphorylase B (97.2 kDa), were added to sterile distilled 639 water (SDW) or B. subtilis culture supernatant at T2 (Culture) to the concentration of 0.9 μg/μl. ..

    Concentration Assay:

    Article Title: Intercellular signal transduction within the mother cell compartment during Bacillus subtilis sporulation.
    Article Snippet: .. 637 a; Protein molecular weight markers (Takara Bio Inc.), including pig myosin (200 kDa), E. coli β-638 galactosidase (116 kDa), and rabbit muscle phosphorylase B (97.2 kDa), were added to sterile distilled 639 water (SDW) or B. subtilis culture supernatant at T2 (Culture) to the concentration of 0.9 μg/μl. ..

    Cloning:

    Article Title: Enhanced extracellular production of maltotetraose amylase from Pseudomonas saccharophila in Bacillus subtilis through regulatory element optimization
    Article Snippet: The codon-optimized gene encoding Mta (GenBank accession number E02567.1) from Pseudomonas saccharophila was synthesized by Synbio Technologies (Suzhou, China). .. The PrimeSTAR HS DNA polymerase, In-Fusion HD Cloning Kit, E. coli HST08 Premium Competent Cells, DNA and protein molecular weight markers were purchased from Takara (Dalian, China). .. Maltotetraose standard (HPLC ≥ 98%) was purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China).



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